Manufactured and quality-controlled at New England Biolabs Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq. Robust reactionsmaximal success with minimal optimization. Offered with multiple buffers for customized reaction setup for different DNA templates.
2020-7-25 · NEB Tm Calculator. Product Group. Q5 Q5 Hot Start Q5U Hot Start OneTaq OneTaq Hot Start Hot Start Taq Taq DNA Polymerase LongAmp Taq LongAmp Hot Start Taq Hemo KlenTaq Vent Deep Vent Master Mix Phusion Phusion Hot Start Flex EpiMark Hot Start. Polymerase/Kit. Q5 High-Fidelity DNA Polymerase Q5 High-Fidelity 2X Master Mix. Primer Concentration
For any questions please use our Phusion Request for Technical Support or call NEB Technical Support at 1-800-632-7799. Phusion® is a registered trademark and property of Thermo Fisher Scientific. Fidelity assays were performed using a lacI-based method modified from Frey Suppman 1995.
Phusion High-Fidelity PCR Master Mix with GC Buffer offers robust performance and can be used for all PCR applications. It is a 2X master mix consisting of Phusion DNA Polymerase deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template primers and water.
Manufactured and quality-controlled at New England Biolabs Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance and thus can be used for all PCR applications. Phusion® High-Fidelity DNA Polymerases Phusion® High-Fidelity DNA Polymerases Phusion® High-Fidelity PCR Master Mix with HF Buffer
Phusion Plus DNA PCR 60°C —— PCR
2017-8-18 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion PCR for mTAG-BFP2 from 25 ng plasmid. Overall it worked if I m being picky there seems to be a bit more smearing in the homemade buffer however the amount of desired product is the same between the two buffers.
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates. Additionally Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template primers and water. Fidelity assays were performed using a lacI-based method modified from Frey Suppman
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates. Additionally Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
For any questions please use our Phusion Request for Technical Support or call NEB Technical Support at 1-800-632-7799. Phusion® is a registered trademark and property of Thermo Fisher Scientific. Fidelity assays were performed using a lacI-based method modified from Frey Suppman 1995.
2017-8-18 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion PCR for mTAG-BFP2 from 25 ng plasmid. Overall it worked if I m being picky there seems to be a bit more smearing in the homemade buffer however the amount of desired product is the same between the two buffers.
While NEB develops and validates its products for various applications the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. PHUSION® is a registered trademark and property of Thermo Fisher Scientific.
Manufactured and quality-controlled at New England Biolabs Thermo Scientific ® Phusion ® High-Fidelity DNA Polymerase offers both high fidelity and robust performance and thus can be used for all PCR applications. Its unique structure a novel Pyrococcus -like enzyme fused with a processivity-enhancing domain increases fidelity and speed.
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme even on your most difficult templates. Additionally Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
2021-6-23 · Choose a DNA RNA genome editing qPCR calculator from NEB a leader in production and supply of reagents for the life science industry.
Annealing temperatures required for use with Phusion tend to be higher than with other PCR polymerases. The NEB Tm calculator should be used to determine the annealing temperature when using Phusion. Typically primers greater than 20 nucleotides in length anneal for 10–30 seconds at 3°C above the T m of the lower T m primer.
Phusion Hot Start Flex DNA Polymerase is available as standalone enzyme or in a master mix format and enables high specificity amplification of a broad range of templates. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. NEB offers a guidelines for choosing the correct DNA polymerase for
This page presents all the information in Polbase for Phusion Hot Start HF. Mutants At the top of the page you ll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section. Structures/Sequence If available a representative structure is
Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error-rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1) Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity 3´→ 5´
Phusion. GC Buffer Pack. New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products all of which are manufactured and quality controlled at New England Biolabs.
2017-8-18 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion PCR for mTAG-BFP2 from 25 ng plasmid. Overall it worked if I m being picky there seems to be a bit more smearing in the homemade buffer however the amount of desired product is the same between the two buffers.
For any questions please use our Phusion Request for Technical Support or call NEB Technical Support at 1-800-632-7799. Phusion® is a registered trademark and property of Thermo Fisher Scientific. Fidelity assays were performed using a lacI-based method modified from Frey Suppman 1995.
Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer as well as 5X Phusion GC Buffer which can be used for complex or GC-rich templates.
2020-8-12 · The Phusion™ Site-Directed Mutagenesis Kit contains reagents for a total of 20 mutagenesis reactions (including control reactions) and control plasmid and primers for 10 reactions. Component Amount Storage Phusion™ Hot Start II DNA Polymerase 2 U/μL 10 µL −20°C 5X Phusion™ HF Buffer 1.5 mL dNTP Mix 10 mM each 20 µL FastDigest
2017-8-18 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion PCR for mTAG-BFP2 from 25 ng plasmid. Overall it worked if I m being picky there seems to be a bit more smearing in the homemade buffer however the amount of desired product is the same between the two buffers.
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