2020-2-11 · ClonExpress II one step cloning kit (Vazyme). DNA used in the experiments was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific USA). The antibiotics and the concentrations used in culturing B. subtilis were kanamycin (50 μg/ml) chloromycetin (15 μg/ml) ampicillin (100 μg/ml) isopropy-β-D-thiogalactopyranoside (IPTG 0
2021-7-22 · ClonExpress II One Step Cloning Kit C112 Vazyme 6001000 ClonExpress MultiS One Step Cloning Kit Vazyme 5201080
ClonExpress® Ultra One Step Cloning Kit 1-5 C115 800/1300 1183
2021-6-12 · SmUxe was further introduced into the BamHI site of pCZ192-1 and pCZ192-2 via ClonExpress II One Step Cloning Kit to accomplish pCZ193-1 and pCZ193-2. The cassette of SmUxs1-SmUxe was amplified using pCZ192-1 as template and inserted into the Not I site of pCZ165 (pET28a harboring OsUGT708A40 Additional file 1 Table S1) to accomplish pCZ194
ClonExpress® Ultra One Step Cloning Kit 1-5 C115 800/1300 1170
2021-6-22 · Promoter sequences of PsUFGT (2523bp) and PsGST (2339bp) were amplified and inserted into pGreenII 0800–LUC vector using ClonExpress Ultra One Step Cloning Kit (Vazyme Nanjing China). A fragment of PsMYB10.2 (296–645 bp) was amplified using specific primers (TRV2-PsMYB10.2F and pTRV2-PsMYB10.2R) and inserted into pTRV2 to generate pTRV2
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
2019-10-15 · VAZYME C115-ClonExpress Ultra One Step Cloning Kit pBluescript II SK( )T7infusion pBluescript II SK( )T7terminator pBluescript II SK( )T7terminatorKpn1Not1 P
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector
ClonExpress II One Step Cloning Kit ClonExpress Ultra One Step Cloning Kit . 5min Universal Ligation Mix (1 / 1) 1 BioLinks Lab Supplies. Nijenheim 1325. 3704VC Zeist. The Netherlands. Tel 00-31-030-2765796. Email info bio-links. Reviews. Rating 3.6363636363636 stars
C115-02 ClonExpress®Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
2021-7-22 · ClonExpress II One Step Cloning Kit C112 Vazyme 6001000 ClonExpress MultiS One Step Cloning Kit Vazyme 5201080
2020-2-11 · Biolabs (England). ClonExpress II one step cloning kit (Vazyme). DNA used in the experiments was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific USA). The antibiotics and the concentrations used in culturing B. subtilis were kanamycin (50 μg/ml) chloromycetin (15 μg/ml) ampicillin (100 μg/ml) isopropy-
2021-6-12 · SmUxe was further introduced into the BamHI site of pCZ192-1 and pCZ192-2 via ClonExpress II One Step Cloning Kit to accomplish pCZ193-1 and pCZ193-2. The cassette of SmUxs1-SmUxe was amplified using pCZ192-1 as template and inserted into the Not I site of pCZ165 (pET28a harboring OsUGT708A40 Additional file 1 Table S1) to accomplish pCZ194
ClonExpress II One Step Cloning Kit-——-ClonExpress®IIOneStepCloningKit 50bp-10kbDNA
2020-7-28 · ClonExpress Ultra One Step Cloning Kit. 515min . Mix . 50 bp 10 kb >95 .
2020-12-26 · and XhoI (NEB) via ClonExpress Ultra One Step Cloning Kit (Vazyme). After Sanger sequencing 100 mL of overnight E. coli culture harboring the 35S GmJAG1-3Avi plasmid (Fig. S1) was used for plasmid prepa-ration by the Plasmid Maxi Kit (QIAGEN) and the endotoxin was removed by Trixon-114 (Sigma) 18 .
2019-8-21 · A ClonExpress Ultra One Step Cloning Kit (Vazyme China) was utilized to introduce mutations in selected AAV1 or AAV5 capsid residues at the
2021-4-15 · The pegRNA constructs were made as reported previously 8 by amplifying the spacer-scaffold-RT-PBS fragment by one-step PCR and cloning it into OsU3-sgRNA vectors using a ClonExpress II One Step
2021-4-15 · The pegRNA constructs were made as reported previously 8 by amplifying the spacer-scaffold-RT-PBS fragment by one-step PCR and cloning it into OsU3-sgRNA vectors using a ClonExpress II One Step
2020-2-11 · Biolabs (England). ClonExpress II one step cloning kit (Vazyme). DNA used in the experiments was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific USA). The antibiotics and the concentrations used in culturing B. subtilis were kanamycin (50 μg/ml) chloromycetin (15 μg/ml) ampicillin (100 μg/ml) isopropy-
2019-8-21 · A ClonExpress Ultra One Step Cloning Kit (Vazyme China) was utilized to introduce mutations in selected AAV1 or AAV5 capsid residues at the
2020-3-6 · ligated into the pET-28a( ) vector (Novagen) using a ClonExpress Ultra One Step Cloning Kit (Table S3). The intron-free nsrK and nsrQ genes were prepared by amplifying and ligating the two predicted exons in which the sequence of nsrK in the database was revised (Table S5). Since the nsrO gene does not have an intron it
C112-02 ClonExpress® II One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology. Log in . Forgot your password Open account. ClonExpress®Ultra One Step Cloning Kit. 50rxn Wilhelm-Mauser-Str. 41-43 50827 KölnGermany Tel 49 221 9498320
2020-3-6 · ligated into the pET-28a( ) vector (Novagen) using a ClonExpress Ultra One Step Cloning Kit (Table S3). The intron-free nsrK and nsrQ genes were prepared by amplifying and ligating the two predicted exons in which the sequence of nsrK in the database was revised (Table S5). Since the nsrO gene does not have an intron it
2020-3-6 · ligated into the pET-28a( ) vector (Novagen) using a ClonExpress Ultra One Step Cloning Kit (Table S3). The intron-free nsrK and nsrQ genes were prepared by amplifying and ligating the two predicted exons in which the sequence of nsrK in the database was revised (Table S5). Since the nsrO gene does not have an intron it
BQ+ Medical not only produce components for manufacturers all over the world, but also provide private label manufacturing for leading brand distributors in different countries.
8,Cheye Road,Songjiang District,Shanghai 201611 China.
Tel: 86-021-57743953
Email: [email protected]
Copyright © BQ PLUS MEDICAL CO., LTDSitemap | Contact Us |
